Journal: PLoS ONE

Article Title: Lung Fibroblasts Share Mesenchymal Stem Cell Features Which Are Altered in Chronic Obstructive Pulmonary Disease via the Overactivation of the Hedgehog Signaling Pathway

doi: 10.1371/journal.pone.0121579

Figure Lengend Snippet: (a)Rate of lung fibroblast conversion into myofibroblasts after 3, 4 and 5 days exposure to TGF-β1. * p<0.05 compared to untreated cells. # p<0.05 compared to non- smoker (C-NS) and smoker controls (C-S) exposed to TGF-β1 for 4 or 5 days. (b) Oil-red O staining of, MSC, DF and lung fibroblasts from C-NS, C-S and COPD subjects12 days after exposure to adipogenic differentiation medium. (c)Alcian blue staining of MSC, DF and lung fibroblasts at day 24 following chondrogenesis initiation. (d,e) (D, E) Comparative real-time PCR analysis of mRNA expression of genes encoding for (d) adipogenic peroxysome proliferative activated receptor gamma 2 (PPAR-γ 2; early), lipoprotein lipase (LPL; late) and (e)chondrogenic Sox-9 marker. (f) Alizarin red staining of cells following 17 days of osteoblastic induction and (g)real-time PCR analysis of mRNA expression of osteoblastic alkaline phosphatase (ALP, early) and osteocalcin (OC, late) markers. (d,e,g) Results represent means ± SEM of at least 3 independent experiments performed with donor fibroblasts. NS: non significant; * p<0.05, ** p<0.01. MSC, mesenchymal stem cells; DF, dermal fibroblast; C-NS, non-smokers controls; C-S, smoker controls; COPD, smokers with COPD.

Article Snippet: Chondrogenic differentiation was carried out using Mesenchymal Stem Cell Chondrogenic Differentiation Medium (PromoCell, Heidelberg, Germany).

Techniques: Staining, Real-time Polymerase Chain Reaction, Expressing, Marker

Journal: Stem Cell Research & Therapy

Article Title: Myogenic progenitor cell transplantation for muscle regeneration following hindlimb ischemia and reperfusion

doi: 10.1186/s13287-021-02208-w

Figure Lengend Snippet: Cell characterization. a Immunocytochemical staining of MPC and NMC demonstrating positive desmin expression in MPC (red color) and negative desmin expression in NMC. Nuclear counterstaining performed by hematoxylin. b Live-cell imaging of Syto24 stained TdTomato MPCs, cultivated in skeletal muscle differentiation medium for 4 days (blue arrows: multinucleated myotubes). c Quantification of AChE activity per 2 × 10 5 cells in either MPC or NMC both cultivated in skeletal muscle differentiation medium. d Flow cytometric analysis of CD9 and CD98 surface marker in MPC from at least two different mice demonstrated as histogram (red). Isotype control stained cells (white histograms) used to determine threshold for positivity. AChE, acetylcholinesterase; MPC, myogenic progenitor cell; NMC, non-myogenic cells

Article Snippet: Afterwards, the coating solution was aspirated and 125.000 cells in 1 mL were directly seeded and allowed to attach for 24 to 48 h. Afterwards, differentiation was induced by aspirating the growth medium and adding 1 mL skeletal muscle cell differentiation medium (PromoCell, Germany), supplemented with 2% of skeletal muscle cell differentiation medium Supplement Mix (PromoCell, Germany) and 0.05% gentamicin solution (8 mg/mL, Sandoz, Austria).

Techniques: Staining, Expressing, Live Cell Imaging, Activity Assay, Marker